DNA-Based Information Technologies

According to the description of the classic E. coli plasmid pBR322, what is its approximate size in base pairs?

What are the three essential components that define signal transduction through G protein–coupled receptors (GPCRs)?

After 20 cycles of the polymerase chain reaction (PCR), what is the approximate amplification factor for the targeted DNA segment?

What is the typical range of DNA segment length, in base pairs, that can be cloned using Bacterial Artificial Chromosomes (BACs)?

What is the primary biological function of restriction endonucleases in bacteria?

Which enzyme is essential for covalently joining a DNA fragment into a cloning vector?

When a restriction endonuclease makes staggered cuts on the two DNA strands, what are the resulting unpaired strands referred to as?

What is the term for cloning vectors that are specifically designed with transcription and translation signals for the regulated production of a protein from a cloned gene?

What is the primary purpose of using terminal tags, such as a (His)6 tag or a GST tag, on a cloned gene?

What unique enzyme enables the polymerase chain reaction (PCR) to amplify RNA sequences?

A DNA library created from the messenger RNA (mRNA) of an organism is known as a:

What term describes homologous genes found in different species that have a clear sequence and functional relationship?

The green fluorescent protein (GFP), a valuable tool for visualizing proteins in living cells, is naturally derived from which organism?

In a yeast two-hybrid analysis, the interaction of two proteins, X and Y, is detected by the functional reconstitution of which transcription factor?

In a DNA microarray experiment comparing gene expression at two developmental stages using green and red fluorescent cDNA, what does a yellow spot signify?

What is the typical read length, in nucleotides, for the Illumina sequencer, a type of next-generation sequencing method?

Approximately how many base pairs (bp) does the human genome contain?

What is the approximate number of protein-coding genes in the human genome?

In eukaryotic genes, what are the nontranslated DNA segments that interrupt the protein-coding sequences called?

What is the term for a group of SNPs and other genetic variations that are located close together on a chromosome and are typically inherited as a block?

The fusion protein created by joining a target gene to the gene for Glutathione-S-transferase (GST) can be purified using affinity chromatography where the column matrix is immobilized with what ligand?

In the yeast two-hybrid system, what are the two separable domains of the Gal4 protein that are brought together by an interaction between two test proteins?

What is the approximate amplification factor for a DNA segment after 30 cycles of PCR?

What percentage of the human genome consists of protein-coding exon DNA?

Which type of restriction endonuclease, such as BamHI or EcoRI, is most useful for recombinant DNA technology because it cleaves DNA within its specific recognition sequence?